RESULTS OF MICROBIOLOGICAL STUDIES OF SOIL IN SHEEP BRADZOT
DOI:
https://doi.org/10.62724/202530404Keywords:
Вradzot, Clostridium septicum, pathogenicity, bioassay, nutrient medium, soil sample.Abstract
The article presents the results of microbiological studies of soil samples collected from farms where sheep braxy was diagnosed. The research established that the isolation of anaerobic pathogens similar to Clostridium septicum from soil indicates a direct link between the occurrence of the disease in sheep and environmental contamination.
The isolated pathogens, when cultured on nutrient media, exhibited morphological and biochemical characteristics typical of the braxy causative agent. The pathogenicity of the isolates was confirmed through experimental infection of laboratory animals, followed by successful identification of the pathogen. The primary sources of infection are sick animals and the carcasses of deceased sheep. Through their feces, urine, and various secretions, they release Clostridium septicum spores into the environment.
The study results revealed a high level of soil contamination in areas where sheep were kept on pastures, in pens, and in livestock housing. During bacteriological examinations, highly virulent strains of the pathogen were frequently isolated from these locations.
In this regard, farms where a diagnosis of braxy has been confirmed must implement comprehensive disinfection measures in livestock facilities, pasturelands, and burial sites of animal carcasses. Personnel working with contaminated soil and animal remains especially veterinary professionals must strictly adhere to biosafety requirements.
Timely disposal of carcasses and proper sanitation of the environment play a crucial role in preventing epizootics. To identify the factors contributing to the spread of sheep bradzot particularly bacterial carriage and the epizootiological significance of soil special microbiological studies were conducted.
The work was carried out on farms located in the Taskala District of the West Kazakhstan Region, in cases where sheep exhibited clinical and pathological signs of braxy. Soil samples were collected from locations where sick animals had been kept, including barns, pastures, areas near water sources, and livestock driveways. The soil samples were placed in sterile containers and transported to the laboratory under controlled storage conditions. The microbiological studies were conducted at the «Testing Center» of the Non-Profit Joint Stock Company "Zhangir Khan West Kazakhstan Agrarian-Technical University».